However, the differential properties of TSCs and tenocytes remain largely undefined. This study aims to characterize the properties of these tendon cells derived from rabbits. TSCs and tenocytes were isolated from patellar and Achilles tendons of rabbits.
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The differentiation potential and cell marker expression ptsc the two types of cells were examined using histochemical, immunohistochemical, and qRT-PCR analysis as well as in vivo implantation. In addition, morphology, colony formation, and proliferation of TSCs and tenocytes were also compared. It was found fiona gallagher porn TSCs were able to differentiate into adipocytes, chondrocytes, and osteocytes in vitroand form tendon-like, cartilage-like, and bone-like tissues in vivo.
In contrast, tenocytes had little such differentiation potential.
Morphologically, TSCs possessed smaller cell bodies and larger nuclei than ordinary tenocytes and had cobblestone-like morphology in confluent culture whereas tenocytes were highly elongated.
TSCs also proliferated more quickly than tenocytes in culture.
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Additionally, TSCs from nude tendons formed more numerous and larger colonies and proliferated more rapidly than TSCs from Achilles tendons. TSCs exhibit distinct properties compared to tenocytes, including differences in cell marker expression, proliferative and differentiation potential, and cell morphology in culture. Future research should investigate the mechanobiology of TSCs and explore the possibility of using TSCs to more effectively repair or model injured tendons.